Individuals with metabolic syndrome (MetS) often have elevated plasma plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator... » More

Individuals with metabolic syndrome (MetS) often have elevated plasma plasminogen activator inhibitor-1 (PAI-1) and

tissue plasminogen activator (t-PA), contributing to an increased risk of cardiovascular disease. PAI-1 and t-PA may be

affected by chronic (n-3) long-chain PUFA [(n-3)LCPUFA] supplementation; however, the acute impact of fat ingestion on

these risk factors has not been established. Our objective was to investigate the acute effect of (n-3)LCPUFA on plasma

PAI-1, t-PA, and platelet aggregation. We conducted a randomized crossover study in which men (n = 8, $45 y) with MetS

consumed water or a high-saturated fat beverage (1 g fat/kg body weight) with either a high or low content of (n-3)

LCPUFA. Blood samples were collected over 8 h to measure triacylglycerol (TAG), PAI-1, t-PA, and platelet aggregation.

Both fat loads resulted in a signi?cant increase in whole blood TAG concentration, plasma PAI-1 and t-PA concentrations,

and PAI-1 activity, as well as a signi?cant decrease in t-PA activity during the postprandial period. Interestingly, PAI-

1 concentration and activity increased more following the high (n-3)LCPUFA compared with the low (n-3)LCPUFA

beverage (P , 0.05). Furthermore, the high (n-3)LCPUFA beverage resulted in a lower t-PA activity (P , 0.05), whereas

the effects of the 2 fat loads on the plasma t-PA concentration and platelet aggregation did not differ. Overall, acute intake

of a high (n-3)LCPUFA beverage shifted the balance between plasma PAI-1 and t-PA, which might indicate a lower

capacity for ?brinolysis.

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