Vaughan on INSDC submission and Kaye on Sequencing Pipelines at GSC8

submitted by: JGI

Bob Vaughan of EMBL on submitting MIGS/MIMS/MIENS information to EMBL-EBI's system, followed by a brief talk from Jon Kaye of the Gordon and Betty Moore Foundation on standards and the foundation's sequencing pipelines at the Genomic Standards Consortium's 8th meeting at the DOE JGI in Walnut Creek, Calif. on Sept. 9, 2009.

Victor Markowitz on "IMG Systems for Comparative Analysis of Microbial Genomes and Metagenomes"

submitted by: JGI

Victor Markowitz, head of the Biological Data Management and Technology Center at Lawrence Berkeley National Laboratory, talks about the Integrated Microbial Genomes system housed at the DOE JGI at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM on May 29, 2009.

Towards Transparency of Computational Analyses: A Central SOP Repository

submitted by: dougramsey
The methodologies used to generate genome and metagenome annotations are diverse and vary between groups and laboratories. Descriptions of the annotation process are helpful in interpreting genome annotation data. Some groups have produced Standard Operating Procedures (SOPs) that describe the annotation process, but standards are lacking for structure and content of these descriptions. A general structure for SOP documents that are relevant to genome annotation, metagenomics, environmental...

Genomic and Genetic Insight into Gut Microbiota Function and Manipulation

submitted by: dougramsey
Trillions of microbes live in our digestive tract and influence our biology in profound and diverse ways. Several diseases, including obesity and inflammatory bowel diseases, have been associated with large-scale shifts in microbiota composition. The ability to address basic questions concerning community function and plasticity are fundamental to understanding the extent of causal relationships between host biology and microbiota perturbations, and whether the microbiota is a viable...

The Importance of Context for the Design and Interpretation of Comparative Metagenomics Studies: The MINIMESS Proposal

submitted by: dougramsey
Jeroen Raes, European Molecular Biology Laboratory Comparative metagenomics is a powerful tool to detect environment-specific adaptation of the communities that inhabit them. However, a wide range of technical and biological interfering factors hamper the correct interpretation of comparative metagenomics results. Here, I will describe some of these factors and pitfalls and propose a minimal metagenome sequence analysis standard (MINIMESS) to cope with these issues. This suggested...

Extending MIGS/MIMS to the Description of Ribosomal RNA Sequences

submitted by: dougramsey
With the MIGS/MIMS specifications the Genomic Standards Consortium has finished the groundwork to enrich our genome and metagenome collections with contextual data. Now, it is time to consider whether these standards could be applied ‘as is’ in the short-term, and ‘with modification/extension’ in the longer-term to any genetic marker sequence retrieved from the environment. To move for ward and leverage existing interest in the community the proposal for MIENS, the ‘Minimum...

New Insights Into Lignocellulose Conversion by Termite Gut Microbes

submitted by: dougramsey

Termites and their complex hindgut microbiota are able to convert wood lignocellulose into hydrogen and other products used to fuel their metabolisms. Recent gene and genome and metagenome based analyses on the gut community have revealed novel insights into many bacteria-mediated, important symbiotic functions. The system-wide gene analysis of a microbial community specialized towards plant lignocellulose degradation has both basic and applied implications.

Targeted Gene Identification from Short Gene Fragments in Metagenome and their Use in Biogeochemical Studies

submitted by: dougramsey
Metagenomics allows for a new approach to studying biogeochemical processes in an environment, by giving an approximation of a community’s biogeochemical potential through the genes involved in a given process. Lipid biomarkers are well suited to this kind of approach because they are important for a number of biogeochemical questions and some have well studied biosynthetic pathways. One important class of lipid biomarkers, triterpenoids, were investigated in a new metagenomic sample that...

Jason Flowers Metabolic Characterization of Candidatus Accumulibacter Phosphatis Using Metaproteomic Analysis

submitted by: dougramsey
In many freshwater bodies, phosphorus is a limiting nutrient for bacterial growth. To prevent eutrophication, enhanced biological phosphorus removal (EBPR) of waste water has been successfully applied at sewage plants for the past thirty years. Previous researchers could only speculate about the biochemical pathways involved in EBPR principally because the organisms responsible for EBPR cannot be isolated in pure culture. Using metagenomic data recently obtained from a Candidatus...