life science

First Optimal Drug Combination for Roundworms

submitted by: raroian
Intestinal roundworms infect >2 billion of the poorest peoples and have received little attention. Very little money is spent on much needed drug development for human parasites, making them one of the great neglected tropical diseases. Recently, a new and powerful class of anti-roundworm drugs (anthelmintic) has emerged- crystal proteins (e.g., Cry5B) made by Bacillus thuringiensis (Bt). Here we show that combining Cry5B with nicotinic acetylcholine receptor agonists like tribendimidine and...

GST Enzymatic Digestion

submitted by: abnova
By Abnova - GST fusion proteins allow convenient affinity purification of many proteins of interest but the GST-tag may become inconvenient in various downstream applications. We use PreScission protease to digest the GST fusion proteins and the protein without GST-tag is obtained.

Protein Quantification (Silver Stain)

submitted by: abnova
By Abnova - Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity while using very simple and cheap equipment and chemicals. The procedures of silver staining are protein fixation, silver impregnation and image development.

Simon Prochnik on Naegleria gruberi at the DOE JGI

submitted by: JGI
Simon Prochnik spoke about the Naegleria gruberi genome project at the DOE JGI on February 3, 2010.

A sustainable U.S. Mexico Border

submitted by: dgallego12
In this research, we wanted to determine whether it was beneficiary to restore Los Laureles Canyon due to minimal effects done to the region. Understanding the ecological footprint and challenges it introduces to the bio region of Los Laureles Canyon through analyses of different restorations attempts has question whether it is convenient to continue restoration plans.

Cell Culture (Attached Cell)

submitted by: abnova
by Abnova - Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture attached cells from cell thawing, cell maintenance, to cell freezing.