Silencing the Menkes Copper-Transporting ATPase (Atp7a) Gene in Rat Intestinal Epithelial (IEC-6) Cells Increases Iron Flux via Transcriptional Induction of Ferroportin 1 (Fpn1)

submitted by: jfcollins
The Menkes copper-transporting ATPase (Atp7a) gene is induced in rat duodenum during iron deficiency, consistent with copper accumulation in the intestinal mucosa and liver. To test the hypothesis that ATP7A influences intestinal iron metabolism, the Atp7a gene was silenced in rat intestinal epithelial (IEC-6) cells using short hairpin RNA (shRNA) technology. Perturbations in intracellular copper homeostasis were noted in knockdown cells, consistent with the dual roles of ATP7A in pumping...
Authors: Sukru Gulec, James F. Collins

biosights: Decemeber 23, 2013 - Tumor cells WASH away the extracellular matrix

submitted by: JCB
Tumor cells invade through extracellular matrices by forming actin-rich structures called invadopodia, which contain the transmembrane matrix metalloproteinase MT1-MMP. Monteiro et al. reveal that the Arp2/3 activating protein WASH works with the exocyst complex to deliver MT1-MMP from late endosomes to the invadopodial plasma membrane. This biosights episode presents the paper by Monteiro et al. from the December 23, 2013, issue of The Journal of Cell Biology and includes an interview with...

biosights: November 25, 2013 - Caspases work as branch managers

submitted by: JCB
When a growing axon nears its target, it arborizes, or branches, to form numerous synaptic connections. Campbell and Okamoto reveal that localized caspase activation downstream of Slit-Robo signaling promotes arbor dynamics and restricts arbor growth. This biosights episode presents the paper by Campbell and Okamoto from the November 25, 2013, issue of The Journal of Cell Biology and includes an interview with lead author Douglas Campbell (formerly at the RIKEN Brain Science Institute,...

MWV #81 - Sheldon Campbell - The Singing Microbiologist

submitted by: MicrobeWorld
Sheldon Campbell sings about microbiology. Dr. Campbell teaches microbiology at Yale School of Medicine and he uses music to enhance his lectures. He has one song for every block of lectures he gives on a major topic. Songs he's written include a song about fungi, tick borne disease, tuberculosis and one that reviews all of microbiology in eight minutes. Dr. Campbell hasn't done any testing to see his songs are more effective at getting his message across but he does get the occasional...

biosights: October 28, 2013 - A framework for understanding muscle microtubules

submitted by: JCB
The microtubules of skeletal muscle fibers are arranged into an orthogonal grid, but how this network is formed is unknown. Oddoux et al. reveal that the network is built by dynamic microtubules nucleated from Golgi elements. This biosights episode presents the paper by Oddoux et al. from the October 28, 2013, issue of The Journal of Cell Biology and includes an interview with senior author Evelyn Ralston (NIH, Bethesda, MD). Produced by Caitlin Sedwick and Ben Short. See the associated...

biosights: September 30, 2013 - The nodal network

submitted by: JCB
In addition to specialized actin structures such as lamellipodia and stress fibers, cells are thought to contain a contractile actomyosin matrix that maintains cell shape. Luo et al. describe the organization and dynamics of an actomyosin network that may fulfill this function. This network is formed by actin nodes that contain the formin DAAM1 and the crosslinker filamin A, and that are connected to each other by myosin II. This biosights episode presents the paper by Luo et al. from the...