Protein Secretion and Vesicle Traffic: Part 2: Biochemical Reconstitution of Transport Vesicle Budding (25:14)

Secretion mutants that block protein exit from the endoplasmic reticulum define genes involved in the formation,... » More

Secretion mutants that block protein exit from the endoplasmic reticulum define genes involved in the formation, targeting and fusion of a small vesicle intermediate. SEC genes corresponding to the mutants defective in vesicle budding define the cytoplasmic machinery responsible for transport vesicle morphogenesis. A biochemical reaction that reproduces ER vesicle budding was reconstituted with gently-broken yeast cells and pure recombinant Sec proteins required in vivo for this budding event. The Sec proteins assemble on the ER membrane in the presence of GTP which activates a small GTPase, Sar1, initiating the formation of a coat protein complex called COPII. « Hide