Videos in iBioseminars

Protein Secretion and Vesicle Traffic : Part 3: Human Diseases of Vesicle Budding (32:28)
Human COPII genes are duplicated and some may have evolved specialized functions. Two rare human diseases affect the activity of one of two copies of Sar1 and the Sec23A subunit of the COPII coat....

Protein Secretion and Vesicle Traffic: Part 2: Biochemical Reconstitution of Transport Vesicle Budding (25:14)
Secretion mutants that block protein exit from the endoplasmic reticulum define genes involved in the formation, targeting and fusion of a small vesicle intermediate. SEC genes corresponding to the...

Growth Control in Animal Development: Part 2: Cell Number Control (37:13)
In the second segment of my talk, I describe our work on cell number control in the rat oligodendrocyte cell lineage. Cell numbers depend on controls on both cell death and cell proliferation. We...

Separating Duplicated Chromosomes in Preparation for Cell Division: Part 3: Moving the Chromosomes to the Spindle Poles: the Mechanisms of Anaphase A (41:27)
The third lecture presents evidence, largely from McIntosh's lab, that shows how microtubule depolymerization can move chromosomes in vitro and explores the nature of some of the protein complexes...

Separating Duplicated Chromosomes in Preparation for Cell Division: Part 2: Understanding Mitosis through Experimentation (39:02)
The second lecture describes some key experiments showing the dynamics of a formed mitotic spindle and the ways these may contribute to accurate chromosome motion. Experiments that reveal aspects...

Membrane Rafts: Part 3: Making Rafts in Living Cell Membranes (20:39)
In this lecture, I explore new ideas about how lipid components of the plasma membrane may be organized by active processes, rather than by passive mechanisms involving thermodynamic phase...

Membrane Rafts: Part 2: Looking for Functional Rafts in Cell Membranes (41:55)
In this part, I focus on our laboratory's ongoing preoccupation with rafts where we study the characteristics a popular "raft-marker", a class of cell surface lipid-tethered proteins, the...

Genes and Cells that Determine the Lifespan of C. elegans - Part 2: The Regulation of Aging by Signals from the Reproductive System, and, also, a Link Between Aging and Tumor Growth (37:16)
Once it was thought that aging was just a random and haphazard process. Instead, the rate of aging turns out to be subject to regulation by transcription factors that respond to hormones and other...

Polyketide Biosynthesis: The Erythromycin Example - Part 3: Enzymology of Erythromycin Polyketide Biosynthesis (42:11)
In the final part, the enzymology of the erythromycin polyketide synthase is discussed.

Polyketide Biosynthesis: The Erythromycin Example - Part 2: Impact of Genetics and Molecular Biology on Understanding and Engineering Erythromycin Biosynthesis (22:44)
In the second part, the contributions of genetics and molecular biology on investigations into erythromycin biosynthesis are summarized. The impact of these fields on biosynthetic engineering is...