JCB's Publications

Probing the spindle matrix
A microtubule-independent network of proteins called the spindle matrix is involved in assembling mitotic and meiotic spindles, but whether the matrix makes a mechanical contribution to spindle...

Systems biology of the mitotic spindle
A novel high-throughput screening approach that combines yeast mutant arrays with high-content imaging provides fresh insights into mitotic spindle disassembly. This biosights episode presents the...

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis
Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type–specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not...

DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos
We examined the contribution of S phase in timing cell cycle progression during Drosophila embryogenesis using an approach that deletes S phase rather than arresting its progress. Injection of...

The Dictyostelium type V myosin MyoJ is responsible for the cortical association and motility of contractile vacuole membranes
The contractile vacuole (CV) complex in Dictyostelium is a tubulovesicular osmoregulatory organelle that exhibits extensive motility along the actin-rich cortex, providing a useful model for...

Multiparametric analysis of focal adhesion formation by RNAi-mediated gene knockdown
Cell adhesion to the extracellular matrix is mediated by elaborate networks of multiprotein complexes consisting of adhesion receptors, cytoskeletal components, signaling molecules, and diverse...

Motor-dependent microtubule disassembly driven by tubulin tyrosination
In cells, stable microtubules (MTs) are covalently modified by a carboxypeptidase, which removes the C-terminal Tyr residue of alpha-tubulin. The significance of this selective detyrosination of...

Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis
The establishment of apical–basal polarity within a single cell and throughout a growing tissue is a key feature of epithelial morphogenesis. To examine the underlying mechanisms, the human...

One-Dimensional Topography Underlies Three-Dimensional Fibrillar Cell Migration
Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar...

Collective cell migration requires vesicular trafficking for chemoattractant delivery at the trailing edge
Chemoattractant signaling induces the polarization and directed movement of cells secondary to the activation of multiple effector pathways. In addition, chemotactic signals can be amplified and...