User Profile

ttalley


Name
Todd Talley
Status:
Specialized Professional
Location:
Skaggs School of Pharmacy & Pharmaceutical Sciences University of California, San Diego
Research Interests:
My primary research interests are focused on using molecular pharmacology and medicinal chemistry to investigate structure and function of biomolecules in neurotransmission. Specifically, my research has centered upon the molecular determinants of ligand recognition, catalytic activity and signal transduction.
Publications in PubMed
  1. Utsintong M, Talley TT, Taylor PW, Olson AJ, Vajragupta O. Virtual screening against alpha-cobratoxin. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening 2009 14(9). DOI:10.1177/1087057109344617.
  2. Hibbs RE, Sulzenbacher G, Shi J, Talley TT, Conrod S, Kem WR, Taylor P, Marchot P, Bourne Y. Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor. The EMBO journal 2009 28(19). DOI:10.1038/emboj.2009.227.
  3. Rana BK, Wessel J, Mahboubi V, Rao F, Haeller J, Gayen JR, Eskin E, Valle AM, Das M, Mahata SK, Taupenot L, Stridsberg M, Talley TT, Ziegler MG, Smith DW, Schork NJ, O'Connor DT, Taylor P. Natural variation within the neuronal nicotinic acetylcholine receptor cluster on human chromosome 15q24: influence on heritable autonomic traits in twin pairs. The Journal of pharmacology and experimental therapeutics 2009 331(2). DOI:10.1124/jpet.109.157271.
  4. Tomizawa M, Talley TT, Park JF, Maltby D, Medzihradszky KF, Durkin KA, Cornejo-Bravo JM, Burlingame AL, Casida JE, Taylor P. Nicotinic agonist binding site mapped by methionine- and tyrosine-scanning coupled with azidochloropyridinyl photoaffinity labeling. Journal of medicinal chemistry 2009 52(12). DOI:10.1021/jm900153c.
  5. Babakhani A, Talley TT, Taylor P, McCammon JA. A virtual screening study of the acetylcholine binding protein using a relaxed-complex approach. Computational biology and chemistry 2009 33(2). DOI:10.1016/j.compbiolchem.2008.12.002.
  6. Talley TT, Harel M, Hibbs RE, Radic Z, Tomizawa M, Casida JE, Taylor P. Atomic interactions of neonicotinoid agonists with AChBP: molecular recognition of the distinctive electronegative pharmacophore. Proceedings of the National Academy of Sciences of the United States of America 2008 105(21). DOI:10.1073/pnas.0802197105.
  7. Tomizawa M, Maltby D, Talley TT, Durkin KA, Medzihradszky KF, Burlingame AL, Taylor P, Casida JE. Atypical nicotinic agonist bound conformations conferring subtype selectivity. Proceedings of the National Academy of Sciences of the United States of America 2008 105(5). DOI:10.1073/pnas.0711724105.
  8. Tomizawa M, Maltby D, Talley TT, Durkin KA, Medzihradszky KF, Burlingame AL, Taylor P, Casida JE. Atypical nicotinic agonist bound conformations conferring subtype selectivity. Proceedings of the National Academy of Sciences of the United States of America 2008 105(5). DOI:10.1073/pnas.0711724105.
  9. Tomizawa M, Maltby D, Medzihradszky KF, Zhang N, Durkin KA, Presley J, Talley TT, Taylor P, Burlingame AL, Casida JE. Defining nicotinic agonist binding surfaces through photoaffinity labeling. Biochemistry 2007 46(30). DOI:10.1021/bi700667v.
  10. Tomizawa M, Talley TT, Maltby D, Durkin KA, Medzihradszky KF, Burlingame AL, Taylor P, Casida JE. Mapping the elusive neonicotinoid binding site. Proceedings of the National Academy of Sciences of the United States of America 2007 104(21). DOI:10.1073/pnas.0703309104.
  11. Kang TS, Radi? Z, Talley TT, Jois SD, Taylor P, Kini RM. Protein folding determinants: structural features determining alternative disulfide pairing in alpha- and chi/lambda-conotoxins. Biochemistry 2007 46(11). DOI:10.1021/bi061969o.
  12. Talley TT, Olivera BM, Han KH, Christensen SB, Dowell C, Tsigelny I, Ho KY, Taylor P, McIntosh JM. Alpha-conotoxin OmIA is a potent ligand for the acetylcholine-binding protein as well as alpha3beta2 and alpha7 nicotinic acetylcholine receptors. The Journal of biological chemistry 2006 281(34). DOI:10.1074/jbc.M602969200.
  13. Talley TT, Yalda S, Ho KY, Tor Y, Soti FS, Kem WR, Taylor P. Spectroscopic analysis of benzylidene anabaseine complexes with acetylcholine binding proteins as models for ligand-nicotinic receptor interactions. Biochemistry 2006 45(29). DOI:10.1021/bi060534y.
  14. Bourne Y, Hansen SB, Sulzenbacher G, Talley TT, Huxford T, Taylor P, Marchot P. Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein. Journal of molecular neuroscience : MN 2006 30(1-2). DOI:10.1385/JMN:30:1:103.
  15. Bourne Y, Talley TT, Hansen SB, Taylor P, Marchot P. Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors. The EMBO journal 2005 24(8). DOI:10.1038/sj.emboj.7600620.
  16. Hibbs RE, Talley TT, Taylor P. Acrylodan-conjugated cysteine side chains reveal conformational state and ligand site locations of the acetylcholine-binding protein. The Journal of biological chemistry 2004 279(27). DOI:10.1074/jbc.M403713200.
  17. Hansen SB, Talley TT, Radic Z, Taylor P. Structural and ligand recognition characteristics of an acetylcholine-binding protein from Aplysia californica. The Journal of biological chemistry 2004 279(23). DOI:10.1074/jbc.M402452200.
  18. Taylor P, Hansen SB, Talley TT, Hibbs RE, Radi? Z. Contemporary paradigms for cholinergic ligand design guided by biological structure. Bioorganicmedicinal chemistry letters 2004 14(8). DOI:10.1016/j.bmcl.2003.10.072.
  19. Gao F, Bern N, Little A, Wang HL, Hansen SB, Talley TT, Taylor P, Sine SM. Curariform antagonists bind in different orientations to acetylcholine-binding protein. The Journal of biological chemistry 2003 278(25). DOI:10.1074/jbc.M301151200.
  20. Hansen SB, Radic' Z, Talley TT, Molles BE, Deerinck T, Tsigelny I, Taylor P. Tryptophan fluorescence reveals conformational changes in the acetylcholine binding protein. The Journal of biological chemistry 2002 277(44). DOI:10.1074/jbc.C200462200.
  21. Doorn JA, Schall M, Gage DA, Talley TT, Thompson CM, Richardson RJ. Identification of butyrylcholinesterase adducts after inhibition with isomalathion using mass spectrometry: difference in mechanism between (1R)- and (1S)-stereoisomers. Toxicology and applied pharmacology 2001 176(2). DOI:10.1006/taap.2001.9279.
  22. Doorn JA, Talley TT, Thompson CM, Richardson RJ. Probing the active sites of butyrylcholinesterase and cholesterol esterase with isomalathion: conserved stereoselective inactivation of serine hydrolases structurally related to acetylcholinesterase. Chemical research in toxicology 2001 14(7). DOI:10.1006/taap.2001.9279.
  23. Doorn JA, Gage DA, Schall M, Talley TT, Thompson CM, Richardson RJ. Inhibition of acetylcholinesterase by (1S,3S)-isomalathion proceeds with loss of thiomethyl: kinetic and mass spectral evidence for an unexpected primary leaving group. Chemical research in toxicology 2000 13(12). DOI:10.1006/taap.2001.9279.
  24. Jianmongkol S, Marable BR, Berkman CE, Talley TT, Thompson CM, Richardson RJ. Kinetic evidence for different mechanisms of acetylcholinesterase inhibition by (1R)- and (1S)-stereoisomers of isomalathion. Toxicology and applied pharmacology 1999 155(1). DOI:10.1006/taap.1998.8608.
Publications
Talley, T.T.J., S.; Richardson, R.; Thompson, C. M., Isomalathion Stereoisomers: Insecticide Impurities or New Probes of Cholinesterase, in Cholinesterases '98, B.P.Q. Doctor, D. and Taylor, P., Editor. 1998, Plenum Press. p. 531-538
History of Education and Employment
Education

University California, San Diego, La Jolla, CA, US
Postdoctoral training in Molecular Pharmacology, 2006

University of Montana, Missoula, MT, US
Ph.D. in Organic Chemistry
Thesis advisor: Professor Charles M. Thompson, 2001

University of Utah, Salt Lake City, UT, US
B.S. in Chemistry (Professional Interdisciplinary), 1995

Positions Held

Visiting International Lecturer
School of Pharmacy and Pharmaceutical Chemistry, Mahidol University
Bangkok 10400, Thailand
Spring 2008

Project Scientist
Skaggs School of Pharmacy and Pharmaceutical Sciences, University California, San Diego
La Jolla, CA 92093-0636
2007-present

Post-graduate Researcher
Department of Pharmacology, University of California, San Diego
La Jolla, CA 92093-0636
2005-2007

Post-doctoral Fellow
Department of Pharmacology, University of California, San Diego
La Jolla, CA 92093-0636
2001-2005

Graduate Research and Teaching Assistant
Department of Chemistry, University of Montana
Missoula, MT 59812
1995-2001