User Profile


Lesley Mathews
Staff Scientist
National Chemical Genomics Center
About mathewsla:
Staff Scientist working on drug screening for orphan diseases.
Publications in PubMed
History of Education and Employment
Research Associate Scientist National Chemical Genomics Center, National Institutes of Health, Rockville, MD.
(December 2010-Current)

Sallie Rosen Kaplan Women in Cancer Research Post-doctoral Fellow-National Cancer Institute-Frederick, MD.
(July 2008-November 2010)
Post-doctoral training in the Laboratory of Cancer Prevention /Cancer Stem Cell Section under the direction of Dr. William Farrar, Ph.D. Microarray profiling (gene expression and promoter tiling arrays) of cancer stem cells (CSCs) isolated from established/primary prostate , pancreatic and kidney cancer cell lines. Characterization/validation of targets isolated from array data. Investigating epithelial-to-mesenchymal (EMT) transition and CSCs as a model for cancer metastasis using an in vitro Matrigel model and in vivo models using luciferase tagged cells. Techniques include: Performing and analyzing microarrays/promoter tiling arrays using Agilent based systems. Axon GenePix Pro software for scanning and gridding arrays, Agilent Feature Extraction, DNA Analytics, Genomic Workbench, Cluster, TreeView, NCI mAdb μArray, Oncomine, Gene Expression Omnibus, and Ingenuity software suites used for analysis. Taq-man based qRT-PCR, Methylation-specific PCR, FACS, Westerns and IPs, and Infrared Antibody detection using the Odyssey scanner (In-Cell Westerns and immunocytochemistry of CSCs). Viral delivery of siRNA and engineering of cell lines.

Graduate Student-Pioneer Valley Life Sciences Institute/PhD candidate in the Molecular and Cellular Biology Program-University of Massachusetts at Amherst, Amherst, MA.
(August 2003-May 2008)
Techniques include: Planning and conducting experiments directly pertaining to my dissertation topic, “Hormones, growth factors, and the regulation of tumor suppressor pathways involved in parity-induced protection of breast cancer.” Direct husbandry and genotyping of mice, procurement and DNA/RNA extraction from fresh human tissue, and mammalian cell culture for experimental treatments. In addition: SYBR based qRT- PCR, microarray analysis, pyrosequencing, siRNA technology and cloning, fluorescent activated cell sorting, MTS based proliferation assays, immunohistochemistry/immunocytochemistry, processing/embedding/sectioning of paraffin embedded tissue, handling of radioactive substances (P32 and H3), fluorescence microscopy, and trained to work in a BSL2 environment. Supervision of 3 student projects: 2 undergraduate and 1 masters level.

Teaching Assistant Molecular and Cellular Biology- University of Massachusetts at Amherst, Amherst, MA.
(September 2003-January 2004)
Techniques include: Conducting weekly review sessions based on questions from students and discussions based on weekly review sheets personally designed. Grading of all assignments and exams. Holding defined as well as personalized office hours.

Molecular Biology Research Associate I-Cetek Corporation, Marlboro, MA.
(December 2002-August 2003)
Techniques include: Developing an extensive library of microorganisms for extraction of their secondary natural products. Extraction of DNA from microorganisms, such as fungi and actinomyctes, for speciation/dereplication using Restriction Fragment Length Polymorphism (RFLP) analysis. Development of a biological assay using the cellular slime mold Dictyostelium discoideum to test if compounds isolated from natural extracts might be mutagenic or cytotoxic.
In addition: Strong background in microscopy, PCR, capillary electrophoresis, and cell culture techniques.

Natural Products Laboratory Associate-Cetek Corporation, Marlboro, MA.
(May 2002-December 2002)
Techniques include:. Extraction of natural products from microorganisms such as fungi and actinomyctes. Analysis of extracts using LC/MS Hewlett Packard 1100 for novel compounds and dereplication. In addition: Running assay robot Tecan ( Model: Genesis RSP 150) to prepare and store extract samples.

Forensic Laboratory Technician-Secon: The Drug Testing Company. Worcester, MA.
(January 2002-May 2002)
Techniques include: Preparation of organic derivatives using solid/liquid extractions, as well as liquid/liquid extractions from human samples for the identification of narcotics. In addition: Hewlett Packard 5890 GC/MS, Thin Layer Chromatography using Toxi-Lab design assay, classification of drugs and drug families, assisting clients and police, as well as lawyers with information about the abused drugs, WinTox software.

Protein Purification and Vaccine Formulation Laboratory Technician-Shire Biologics, INC., Northboro, MA.
(May 2000-January 2002)
Techniques include: Large and small batch purification of recombinant proteins expressed in e.coli (potential vaccine candidates). Formulation of vaccines for injection, aseptic techniques, pilot plant and manufacturing assistance (under GMP conditions), sterile filling of vaccines, protein assay development, SDS-PAGE gels, IEF gels, western blots, silver staining, high performance liquid chromatography (HPLC-Hewlett Packard 1100), column chromatography, analysis of protein secondary structure-fourier transform infrared spectroscopy (FTIR) and