abnova's videos (110)


2024
Apr 26, 2010
Plasmid DNA Extraction (Miniprep)

Abnova - There are several ways to purify plasmids. These methods are often called “minipreps” because of...

1576
Apr 19, 2010
mRNA Extraction

Abnova - Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a...

7741
Apr 19, 2010
Real-time PCR

Abnova - Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously...

5240
Apr 19, 2010
Reverse Transcription PCR

Abnova - Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is...

2074
Apr 19, 2010
RNA Extraction from Cell Culture

Abnova - The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification....

1666
Apr 19, 2010
RNA Extraction from Tissue

Abnova - The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification....

1815
Apr 12, 2010
Gateway Recombination

Abnova - The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. In...

2046
Apr 12, 2010
Cre-Lox Recombination

Abnova - Cre-Lox recombination is a special type of site-specific recombination. The Cre protein is a site-specific...

2503
Mar 29, 2010
PCR Cloning (V)- Colony Selection

Abnova - Colony PCR is useful to determining whether or not a specific colony on a plate has plasmid inserts....

2438
Mar 29, 2010
PCR Cloning (IV)- Transformation

Abnova - The purpose of transformation is to introduce a foreign plasmid into bacteria and the bacteria will...

2277
Mar 29, 2010
PCR Cloning (III)- Ligation

Abnova - DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3 hydroxyl...

2260
Mar 29, 2010
PCR Cloning (II)- Digestion

Abnova - Digestion is the process of cutting DNA molecules with restriction endonucleases. These special enzymes...

2749
Mar 22, 2010
PCR Cloning (I)- Insert PCR

Abnova - The procedure of PCR cloning includes DNA preparation, digestion, ligation and transformation. This video...

1563
Mar 22, 2010
PCR Purification

Abnova - Following PCR, you need to get rid of excess short primers, dNTPs, enzymes, short-failed PCR products and...