Human Cell-Free Expression System Generates Stable-Isotope-Labeled Protein Standards for Mass Spec

submitted by: Chrom Solutions

John Rogers presents his scientific poster about human cell-free expression system for the production of stable-isotope-labeled (heavy) proteins using HeLa cell lysates. Six different stable-isotope-labeled proteins were produced. Isotope incorporation efficiency of greater than 95% was observed for IVT reactions.

Randomized controlled trial of 4 compared with 6 mo of exclusive breastfeeding in Iceland: differences in breast-milk intake by stable-isotope probe.

submitted by: jonathanwells
BACKGROUND: The WHO recommends exclusive breastfeeding (EBF) for 6 mo after birth. However, the time at which breast milk ceases to provide adequate energy and nutrition, requiring the introduction of complementary foods, remains unclear. Most studies that investigated this issue were observational and potentially confounded by variability in social circumstances or infant growth. OBJECTIVE: We hypothesized that EBF infants would consume more breast milk at age 6 mo than infants receiving...
Authors: Jonathan Wells, Olof Jonsdottir, Patricia Hibberd, Mary Fewtrell, Inga Thorsdottir, Simon Eaton, Alan Lucas, Geir Gunnlaugsson, Ronald Kleinman