Translation Workflow Development using Mass Spectrometry

submitted by: Chrom Solutions
Mary Lopez, Ph.D. discusses translational workflows developed by the BRIMS center, designed to address all aspects of proteomics, from biomarker discovery to developing more routine applications using mass spectrometry. The workflows can be divided into two different types-- discovery and targeted. High-resolution mass spectrometry instruments are used during the discovery phase to analyze complex biological samples in an effort to uncover new biomarkers by looking at differential abundance....

The Tranche data repository: Progress made and lessons learned from 24.4 TB of data, 1,612 users and 12,458 depositions.

submitted by: ucsd_idash
ABSTRACT High-throughput data production in proteomics and other post-genome disciplines has resulted in many data sharing, annotation, and dissemination challenges. Successful systems require as much thought and effort be put into the social aspects of engineering as the software side. Close relationships with data generators and users are crucial as is the recognition that development of a database to support a static field is quite different from research fields where the data types,...

New NanoESI Source for Increased NanoLC-MS Performance in a Plug-and-Spray Configuration

submitted by: Chrom Solutions

Reiko Kiyonami presents her scientific poster about the performance of a new nanospray ion source for increased NanoLC-MS performance with the Thermo Scientific EASY-Spray column, a plug-and-spray configuration. Excellent chromatographic separation with ultra-sharp peaks were achieved, as well as retention-time reproducibility observed over a wide range of flow rates and column temperatures, highlighting the robustness, sensitivity and stability of the system.

Detection of rHuEPOs in Horse Plasma Samples at Low pgmL Levels using High-Resolution MS Strategies

submitted by: Chrom Solutions

Madalina Oppermann presents her scientific poster about improving the detection and quantification limits for the analysis of recombinant human erythropoietin (rHuEPOs) in horse plasma for doping control using the Thermo Scientific Q Exactive mass spectrometer. The results show the promise of nano-LC-MS/MS analysis on a high-resolution Orbitrap mass analyzer confirm and quatify the rHuEPO target peptides in a single analytical run, with high specificity, sensitivity and resolution.

Human Cell-Free Expression System Generates Stable-Isotope-Labeled Protein Standards for Mass Spec

submitted by: Chrom Solutions

John Rogers presents his scientific poster about human cell-free expression system for the production of stable-isotope-labeled (heavy) proteins using HeLa cell lysates. Six different stable-isotope-labeled proteins were produced. Isotope incorporation efficiency of greater than 95% was observed for IVT reactions.

Rapid Proteomics Analysis Algorithm Development Using Thermo Scientific Proteome Discoverer Software

submitted by: Chrom Solutions

Iman Mohtashemi presents his scientific poster about node-based framework for proteomics data processing using Thermo Scientific Proteome Discoverer software and a hybrid ion trap-OribtrapTM mass spectrometer. This allows rapid deployment of custom algorithms without the need to develop graphical user interfaces and other input/output infrastructure saving you time.

Higher Resolution Improves Top-Down Protein Identification on Orbitrap Mass Spec for Large Proteins

submitted by: Chrom Solutions

David Horn presents his scientific poster on the necessity for high mass resolution (>200,000) for confidence in identification of proteins above 40kDa using yeast enolase. The comparison between various resolution settings for both direct infusion and LC-MS/MS data are shown, using the Thermo Scientific Orbitrap mass spectrometer to improve confidence and sequence coverage for identification of larger proteins.

Comparative Bioinformatic Analysis of Proteome and Transcriptome Derived from a Single Cell Type

submitted by: Chrom Solutions

Christian Ravnsborg presents his scientific poster on HeLa cell analysis and a new development in web-based software, Thermo Scientific ProteinCenter – an integration with Cytoscape® for visualization of molecular interaction networks. He discusses overcoming the limitations of peptide-centric proteomics and covering a large number of functional pathways.

WIsconsin CEGS Idea

submitted by: MCW_BBC

The Wisconsin Center of Excellence in Genomics Science

Tip of the Week: the New SIB Bioinformatics Resource Portal

submitted by: OpenHelix

For more information about this resource see our blog post at URL In this movie I'll introduce you to the newly expanded ExPASY resource, which is now the new SIB (Swiss Institute of Bioinformatics) Bioinformatics Resource Portal.