PCR Cloning (III)- Ligation

submitted by: abnova

Abnova - DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3 hydroxyl ends of one nucleotide with the 5 phosphate end of another. In PCR cloning, insert DNA and vector digested by the same restriction enzyme can be ligated by DNA ligase. For more videos, visit Abnova!