Octanoate and Nonanoate Oxidation Increases 50-80% Over the First Two Days of Life in Piglet Triceps Brachii and Gracilis Muscle Strips

submitted by: Thomas D. Crenshaw
An in vitro muscle strip incubation system was developed to measure the rate of catabolism of 1 mmol/L [1-14C]octanoate, 1 mmol/L [1-14C]nonanoate, 1mmol/L [9-14C]nonanoate and 10 mmol/L [U-14C]glucose by measuring the recovery of 14CO2. Muscle strips (13 mm?1.5 mm ~50 mg) were isolated from triceps brachii and gracilis muscles of newborn and 2 d old, small (<950 g) and large (>1450 g) piglets. The position of the 14C label in the substrate affected the rate and amount of recovery in...
Authors: M.A. Tetrick, T.D. Crenshaw, N.J. Benevenga