Dietary Selenium Deficiency Partially Rescues Type 2 Diabetes–Like Phenotypes of Glutathione Peroxidase-1–Overexpressing Male Mice

submitted by: xlei
This study was conducted to determine whether dietary Se deficiency precluded overproduction of glutathione peroxidase-1 (GPX1) activity in mice overexpressing (OE) this gene and thus rescued their type 2 diabetes–like phenotypes. A total of 20 male OE and wild-type (WT) mice were fed an Se-deficient (<0.02 mg/kg) diet or an Se-supplemented (0.3 mg/kg as sodium selenite) diet from 1 to 5 mo of age. Dietary Se deficiency eliminated or attenuated (P < 0.05) genotype differences in...
Authors: Xin Gen Lei, Li Li, Matt Pepper, Carol Roneker, Marko Vatamaniuk, Xi Yan

Octanoate and Nonanoate Oxidation Increases 50-80% Over the First Two Days of Life in Piglet Triceps Brachii and Gracilis Muscle Strips

submitted by: Thomas D. Crenshaw
An in vitro muscle strip incubation system was developed to measure the rate of catabolism of 1 mmol/L [1-14C]octanoate, 1 mmol/L [1-14C]nonanoate, 1mmol/L [9-14C]nonanoate and 10 mmol/L [U-14C]glucose by measuring the recovery of 14CO2. Muscle strips (13 mm?1.5 mm ~50 mg) were isolated from triceps brachii and gracilis muscles of newborn and 2 d old, small (<950 g) and large (>1450 g) piglets. The position of the 14C label in the substrate affected the rate and amount of recovery in...
Authors: M.A. Tetrick, T.D. Crenshaw, N.J. Benevenga

The R403Q Myosin Mutation Implicated in Familial Hypertrophic Cardiomyopathy Causes Disorder at the Actomyosin Interface

submitted by: apryl
Background Mutations in virtually all of the proteins comprising the cardiac muscle sarcomere have been implicated in causing Familial Hypertrophic Cardiomyopathy (FHC). Mutations in the β-myosin heavy chain (MHC) remain among the most common causes of FHC, with the widely studied R403Q mutation resulting in an especially severe clinical prognosis. In vitro functional studies of cardiac myosin containing the R403Q mutation have revealed significant changes in enzymatic and...
Authors: Dorit Hanein, Larnele Hazelwood, Susan Lowey, HongJun Lui, Kathleen M. Trybus, Niels Volkmann

Dissection -01- Skin and subcutaneous tissue of trunk and up

submitted by: scivee-team

Dissection of a human body: skin and subcutaneous tissue of trunk and upper thigh. Warning: content may be unsuitable for the faint hearted.