Human Cell-Free Expression System Generates Stable-Isotope-Labeled Protein Standards for Mass Spec

submitted by: Chrom Solutions

John Rogers presents his scientific poster about human cell-free expression system for the production of stable-isotope-labeled (heavy) proteins using HeLa cell lysates. Six different stable-isotope-labeled proteins were produced. Isotope incorporation efficiency of greater than 95% was observed for IVT reactions.

Rapid Proteomics Analysis Algorithm Development Using Thermo Scientific Proteome Discoverer Software

submitted by: Chrom Solutions

Iman Mohtashemi presents his scientific poster about node-based framework for proteomics data processing using Thermo Scientific Proteome Discoverer software and a hybrid ion trap-OribtrapTM mass spectrometer. This allows rapid deployment of custom algorithms without the need to develop graphical user interfaces and other input/output infrastructure saving you time.

Higher Resolution Improves Top-Down Protein Identification on Orbitrap Mass Spec for Large Proteins

submitted by: Chrom Solutions

David Horn presents his scientific poster on the necessity for high mass resolution (>200,000) for confidence in identification of proteins above 40kDa using yeast enolase. The comparison between various resolution settings for both direct infusion and LC-MS/MS data are shown, using the Thermo Scientific Orbitrap mass spectrometer to improve confidence and sequence coverage for identification of larger proteins.

Comparative Bioinformatic Analysis of Proteome and Transcriptome Derived from a Single Cell Type

submitted by: Chrom Solutions

Christian Ravnsborg presents his scientific poster on HeLa cell analysis and a new development in web-based software, Thermo Scientific ProteinCenter – an integration with Cytoscape® for visualization of molecular interaction networks. He discusses overcoming the limitations of peptide-centric proteomics and covering a large number of functional pathways.

Todd Smith (PerkinElmer) at the 2012 SFAF Meeting

submitted by: JGI

Todd Smith on "The PerkinElmer Omics Laboratory" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

WIsconsin CEGS Idea

submitted by: MCW_BBC

The Wisconsin Center of Excellence in Genomics Science

Scott S. Terhune, Ph.D.

submitted by: MCW_BBC

Scott S. Terhune, Ph.D., an assistant professor in Microbiology and Molecular Genetics and the Biotechnology and Bioengineering Center at the Medical College of Wisconsin, describes his research in proteomics, mass spectrometry and Human Cytomeglavirus (HCMV).

Shama P. Mirza, Ph.D.

submitted by: MCW_BBC

Shama P. Mirza, Ph.D., assistant professor of Biochemistry at the Medical College of Wisconsin in Milwaukee, WI describes her research on proteomics and mass spectrometry.

Wisconsin CEGS -- It Is Not What It Sounds Like

submitted by: MCW_BBC

Dr. Michael Olivier, Director of the Wisconsin Center of Excellence in Genomics Science, and Professor of Physiology at the Medical College of Wisconsin describes this NIH-funded center and its research focus on developing new technology in mass spectrometry and proteomics.

"What Makes Us Fat?"

submitted by: MCW_BBC

Research Day presentation by Dr. Michael Olivier on the genetics of obesity conducted at the Biotechnology & Bioengineering Center at the Medical College of Wisconsin in Milwaukee, WI USA.