Rapid LC-MS Screening and Quantitative Analysis of Pesticides in Food Matrix

submitted by: Chrom Solutions

Charles Yang presents his scientific poster on automated qualitative analysis with enhanced data quality. Using these selective and sensitive methods can provide data for non-targeted, retrospective data analysis without intensive work using a Thermo Scientific Exactive Plus Benchtop Orbitrap Platform.

Increasing Speed of UHPLC-MS Analysis of Vitamin D for Research Use

submitted by: Chrom Solutions

Sarah Fair presents her scientific poster about removing manual pipetting from the analysis of vitamin D metabolites, testosterone and cortisol using the accuracy and precision of the Thermo Scientific Platemate Pipetting Workstation. Using the automated system offered significant time savings and passed all acceptance criteria for the compounds tested.

Human Cell-Free Expression System Generates Stable-Isotope-Labeled Protein Standards for Mass Spec

submitted by: Chrom Solutions

John Rogers presents his scientific poster about human cell-free expression system for the production of stable-isotope-labeled (heavy) proteins using HeLa cell lysates. Six different stable-isotope-labeled proteins were produced. Isotope incorporation efficiency of greater than 95% was observed for IVT reactions.

Higher Resolution Improves Top-Down Protein Identification on Orbitrap Mass Spec for Large Proteins

submitted by: Chrom Solutions

David Horn presents his scientific poster on the necessity for high mass resolution (>200,000) for confidence in identification of proteins above 40kDa using yeast enolase. The comparison between various resolution settings for both direct infusion and LC-MS/MS data are shown, using the Thermo Scientific Orbitrap mass spectrometer to improve confidence and sequence coverage for identification of larger proteins.

Comparative Bioinformatic Analysis of Proteome and Transcriptome Derived from a Single Cell Type

submitted by: Chrom Solutions

Christian Ravnsborg presents his scientific poster on HeLa cell analysis and a new development in web-based software, Thermo Scientific ProteinCenter – an integration with Cytoscape® for visualization of molecular interaction networks. He discusses overcoming the limitations of peptide-centric proteomics and covering a large number of functional pathways.