Development of a solid support from lahar for enzyme immobilization

submitted by: UP Los Baños
A report on the UPLB Basic Research Program-funded study on the immobilization of papain or bromelain by covalent bonding. In this study, the optimum conditions of pH, temperature and substrate concentration for maximum activity of free and immobilized papain and bromelain were determined. The kinetic parameters, Km and Vmax of the free and immobilized enzymes were also determined and compared. Finally the stability of immobilized the enzyme on storage, upon intermittent use and continuous...