A Twin-Track Approach to Building Desmosomes

submitted by: JCB
Desmosomes are intercellular adhesions whose adhesive core is formed by two distinct classes of cadherin molecules -- desmogleins and desmocollins. Nekrasova et al. reveal that these two cadherins are independently transported to the cell surface by two different kinesin motors. This biosights episode presents the paper by Nekrasova et al. from the December 26, 2011, issue of The Journal of Cell Biology and includes an interview with senior author Kathleen Green (Northwestern University...

The Energy Landscape for Molecular Motors - the Kinesin Story

submitted by: icamvid

Jose Onuchic gives a talk at the RPP08 conference on the mechanics of molecular motors. The discussion includes:
1) The reflection of constraints of the molecular architecture the dynamical pathways during stepping
2) The role of rectified diffusional motion.

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis

submitted by: JCB
Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type–specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not known. In this study, we show that the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes required for cargo delivery to maturing melanosomes. In cells depleted of AP-1 or KIF13A, a subpopulation of recycling endosomes...
Authors: Cédric Delevoye, Ilse Hurbain, Danièle Tenza, Jean-baptist Sibarita, Stéphanie Uzan-gafsou, Hiroshi Ohno, Willie Geerts, Arie Verkleij, Jean Salamero, Graça Raposo

Cytoskeletal Motor Proteins by Ron Vale, July 2007 - Part 1: Introduction to Motor Proteins (43:13)

submitted by: video_collector
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools...