CKIδ Decentralizes the Centrosome

submitted by: JCB
When a T cell encounters a target antigen-presenting cell, the lymphocyte's centrosome relocalizes to a specialized contact between the two cells called the immunological synapse. Zyss et al. reveal that casein kinase Iδ helps to reposition the centrosome in activated T cells, perhaps by working with the microtubule plus-end binding protein EB1 to regulate microtubule growth. This biosights episode presents the paper by Zyss et al. from the November 28, 2011, issue of The Journal of Cell...

Role of conserved non-linear structural motifs in the assembly of active protein kinases

submitted by: icamvid

Alexandr Kornev gives a talk at the RPP08 conference that includes:
1) Description of the LSP-alignment
2) Detection of a conserved nonlinear motif in protein kinases
3) Nonlinear motifs termed "spines" anchored to a single helix organize the kinase core.

DFG motif: A Protonation Dependent Conformational Switch

submitted by: icamvid

Yibing Shan gives a talk at the RPP08 conference about the mechanism of DFG flip in Abl kinase:
1) Simulation of DFG flip
2) An energetic model: the role of protonation
3) Experimental test
4) What's the DFG flip for?

Modulation of the Kinase ensemble by Second Messengers

submitted by: icamvid

Michael Wall gives a talk at the RPP08 conference on:
1) Small-angle scattering studies of cGMP-dependent protein kinase
2) The challenge to understand how cellular signaling is controlled by modulation of protein conformational ensembles
3) Diffuse scattering

Dynamics of Large-Scale Domain Motions in Protein Kinase A

submitted by: icamvid

Don Blumenth gives a talk at the RPP08 conference about the application of SAXS small-angle scattering to study the domain motions in Protein Kinase A and other protein kinases specifically in response to cAMP.

Chemical Approaches to Investigating Protein Kinase Autophosphorylation

submitted by: icamvid

Phil Cole gives a talk at the RPP08 conference about:
1) Bisubstrate protein kinase inhibitors and expressed protein ligation
2) Investigation of protein kinase A intramolecular phosphorylation

Understanding Protein Kinase Structure

submitted by: icamvid

NataraJan Kannan discusses understanding protein kinase structure- function relationships using half a million genomic sequences.

Structural Insights into the KEOPS Complex: An Ancient Protein Kinase Containing Machine

submitted by: icamvid

Frank Sicheri talks about an ancient protein kinase containing machine that gives structural insights in the KEOPS complex. This includes the KEOPS structure, and its functions.