Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis

submitted by: JCB
The establishment of apical–basal polarity within a single cell and throughout a growing tissue is a key feature of epithelial morphogenesis. To examine the underlying mechanisms, the human intestinal epithelial cell line Caco-2 was grown in a three-dimensional matrix to generate a cystlike structure, where the apical surface of each epithelial cell faces a fluid-filled central lumen. A discrete apical domain is established as early as the first cell division and between the two daughter...
Authors: Aron Jaffe, Noriko Kaji, Joanne Durgan, Alan Hall

One-Dimensional Topography Underlies Three-Dimensional Fibrillar Cell Migration

submitted by: JCB
Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (µPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and...
Authors: Andrew Doyle, Francis Wang, Kazue Matsumoto