Nuclear envelope starts with a clean sheet

submitted by: JCB
At the start of mitosis, the nuclear envelope and nuclear pore complexes break down and disperse into the endoplasmic reticulum and cytoplasm. Lu et al. use rapid, 3D live cell imaging to reveal that, in contrast to previous models, the nuclear envelope reforms directly from ER cisternae after mitosis and that this happens before nuclear pore complexes start to reassemble. This biosights episode presents the paper by Lu et al. from the August 8, 2011, issue of The Journal of Cell Biology,...

How the ER shapes up and ships out

submitted by: JCB
The endoplasmic reticulum is a complex network of interconnected membrane tubules and cisternae. West et al. use electron tomography to reveal the full diversity of ER structures present in budding yeast and how these structures pass from the mother cell into the bud. This biosights episode presents the paper by West et al. from the April 18, 2011, issue of The Journal of Cell Biology, and includes an interview with senior author Gia Voeltz (University of Colorado, Boulder). Produced by...

Acetylated microtubules let the ER slide

submitted by: JCB
The endoplasmic reticulum is highly dynamic, although little is known about why and how its membrane tubules move. Friedman et al. reveal that ER membranes slide along stable, acetylated microtubules, which might help them contact other organelles such as mitochondria. This biosights episode presents the paper by Friedman et al. from the August 9, 2010 issue of The Journal of Cell Biology, and includes an interview with senior author Gia Voeltz. Produced by Caitlin Sedwick and Ben Short....

Protein Secretion and Vesicle Traffic by Randy Schekman - Part 1: Genetic Dissection of the Secretory Pathway (36:35)

submitted by: video_collector
Protein secretion is executed by a cellular pathway involving the delivery of membrane and soluble secretory proteins in vesicles that capture newly-synthesized proteins assembled in the endoplasmic reticulum (ER) and sorted in the Golgi apparatus. Vesicles fuse with the plasma membrane resulting in the discharge of soluble molecules to the cell exterior and integration of vesicle membrane proteins and lipids in the cell surface. Baker's yeast cells grow by vesicle fusion and secretion at...