Competitive ELISA

submitted by: abnova
Abnova - Competitive ELISA is through competitive binding to measure the amount of analyte. First of all, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, that unbound antibody is removed. The more analyte in the sample, the less antibody will be able to bind to the antigen in the well. The major advantage of a competitive ELISA is the ability to use crude or impure samples...