Multiparametric analysis of focal adhesion formation by RNAi-mediated gene knockdown

submitted by: JCB
Cell adhesion to the extracellular matrix is mediated by elaborate networks of multiprotein complexes consisting of adhesion receptors, cytoskeletal components, signaling molecules, and diverse adaptor proteins. To explore how specific molecular pathways function in the assembly of focal adhesions (FAs), we performed a high-throughput, high-resolution, microscopy-based screen. We used small interfering RNAs (siRNAs) to target human kinases, phosphatases, and migration- and adhesion-related...
Authors: Sabina Winograd-katz, Shalev Itzkovitz, Zvi Kam, Benjamin Geiger

Adhesion, Signaling and Cancer by Mary C. Beckerle, July 2007 - Part 1: Understanding Cancer From Its Beginnings (36:04)

submitted by: video_collector
Cell-substratum adhesion is mediated by integrins, a family of transmembrane, heterodimeric, extracellular matrix receptors that are concentrated at focal adhesions. Integin engagement influences a variety of signaling pathways and regulates cell behaviors including motility, proliferation, and survival. Disturbance of normal integrin function is associated with a variety of pathologic conditions including cancer. In the first segment of my seminar, I provide a broad overview of the cancer...