A diet rich in high glucoraphanin broccoli interacts with genotype to reduce discordance in plasma metabolite profiles through modulating mitochondrial function

submitted by: Andrew Chapple
Background: Observational and experimental studies suggest that diets rich in cruciferous vegetables and glucosinolates may reduce the risk of cancer and cardiovascular disease (CVD). Objective: We tested the hypothesis that a 12-wk dietary intervention with high-glucoraphanin (HG) broccoli would modify biomarkers of CVD risk and plasma metabolite profiles to a greater extent than interventions with standard broccoli or peas. Design: Subjects were randomly assigned to consume 400 g...
Authors: Charlotte Armah, Maria Traka, Jack Dainty, Marianne Defernez, Astrid Janssens, Wing Leung, John Potter, Richard Mithen

Preparation of broccoli extracts for biological studies

submitted by: tkaragiannis
In this video, Katherine Ververis (Epigenomic Medicine, Baker IDI Heart and Diabetes Institute), demonstrates how to extract nutrients from the florets of broccoli. We investigate the effects of broccoli extract on the viability on human erythroleukemic K562 cells. Broccoli evolved ~2000 years ago from a wild cabbage plant in Europe and since the Roman Empire it has been considered a uniquely valuable food among Italians. The word broccoli, is derived from the Italian plural of broccolo,...