We previously determined the effects of dietary selenium (Se) de?ciency or excess on mRNA abundance of 12
selenoprotein genes in pig tissues. In this study, we determined the effect of dietary Se on mRNA levels of the remaining
porcine selenoprotein genes along with protein production of 4 selenoproteins (Gpx1, Sepp1, Selh, and Sels) and body
glucose homeostasis. Weanling male pigs (n = 24) were fed a Se-de?cient (,0.02 mg Se/kg), basal diet supplemented
with 0, 0.3, or 3.0 mg Se/kg as Se-enriched yeast (Angel Yeast) for 16 wk. Although mRNA abundance of the 13
selenoproteins in 10 tissues responded to dietary Se in 3 patterns, there was no common regulation for any given gene
across all tissues or for any given tissue across all genes. Dietary Se affected (P , 0.05) 2, 3, 3, 5, 6, 7, 7, and 8
selenoprotein genes in muscle, hypothalamus, liver, kidney, heart, spleen, thyroid, and pituitary, respectively. Protein
abundance of Gpx1, Sepp1, Selh, and Sels in 6 tissues was regulated (P , 0.05) by dietary Se concentrations in 3 ways.
Compared with those fed 0.3 mg Se/kg, pigs fed 3.0 mg Se/kg became hyperinsulinemic (P , 0.05) and had lower (P ,
0.05) tissue levels of serine/threonine protein kinase. In conclusion, dietary Se exerted no global regulation of gene
transcripts or protein levels of individual selenoproteins across porcine tissues. Pigs may be a good model for studying
mechanisms related to the potential prodiabetic risk of high-Se intake in humans. J. Nutr. doi: 10.3945/jn.112.159020
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