Background: Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is
poorly...
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Background: Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is
poorly characterized. Markers of these processes may provide a deeper understanding of underlying mechanisms.
Objective: We aimed to identify differentially expressed genes in human adipose tissue during weight loss and weight maintenance
after weight loss.
Design: RNA from subcutaneous abdominal adipose tissue from 9 obese subjects was analyzed by using a complementary DNA microarray at baseline after weight loss on a low-calorie diet and after weight maintenance.
Results: Subjects lost 18.8 6 1.8% of weight and maintained this loss during weight maintenance (1.1 6 2.1%; range: 2 9.3 to 10.6%).
Most differentially expressed genes exhibited a reciprocal regulation and returned to baseline after weight loss (2163 genes) and weight maintenance (3175 genes). CETP and ABCG1, both of which participate in the HDL-mediated reverse cholesterol transport (RCT), were among the most upregulated of the 750 genes that were differentially expressed after both processes. Several genes involved in in?ammation were downregulated. The use of real-time polymerase chain reaction con?rmed or partially con?rmed the previously implicated genes TNMD and MMP9 (both downregulated), PNPLA3 (upregulated), and CIDEA and SCD (both reciprocally regulated).
Conclusions: The bene?cial effects of weight loss should be investigated after long-term weight maintenance. The processes of weight
loss and weight maintenance should be viewed as biologically distinct. CETP and ABCG1 may be important mediators of these effects
