Time-resolved monitoring of the transcriptome of MCF-7 breast cancer cells during the emergence of cisplatin resistance

Conference:  ISMB/ECCB 2009

Abstract:  Cisplatin is an emerging new treatment modality of breast cancer, after failure of first line chemotherapy. Development of a resistant phenotype is a major obstacle in clinical cisplatin therapy. In order to understand the mechanisms leading to cisplatin resistance, we investigated the effect of cisplatin on the gene expression profile of MCF-7 cells. Gene expression microarrays were used to generate whole genome expression data. To trace the dynamics of cisplatin resistance development, we used a time series of cDNA microarrays in a way, as reported for lung-cancer [1]. As the emergence of a resistant phenotype is a time-consuming process, we present a long-term kinetic (24 weeks) to follow the time-course of cisplatin resistance manifestation