Translational Proteomics 2.0 Meeting: SEQUEST and TPP Tips

Conference:  "Translational Proteomics 2.0" hosted by Sage-N Research, Inc.

Abstract:  Advanced topics and tips for using SEQUEST and Trans-Proteomic Pipeline tools for proteomics mass spectrometry data analysis. Proteomics researchers will benefit by increasing their sensitivity and decreasing their false discovery rates when identifying proteins and post-translational modifications using proteomics mass spectrometers, particularly the Orbitrap and ion traps.

Key findings:  (1) Semi-tryptic searches are better (2) Use monoisotopic masses for fragment ions [Use monoisotopic masses for precursor ions if data from a high-res instrument] (3) Narrow mass tolerance searches better if search considers precursor mass isotope assignment error