Translational Proteomics 2.0 Meeting: SEQUEST and TPP Tips

July 10, 2009
DOI: 10.4016/11920.01
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Conference:
"Translational Proteomics 2.0" hosted by Sage-N Research, Inc.
Abstract:
Advanced topics and tips for using SEQUEST and Trans-Proteomic Pipeline tools for proteomics mass spectrometry data analysis. Proteomics researchers will benefit by increasing their sensitivity and decreasing their false discovery rates when identifying proteins and post-translational modifications using proteomics mass spectrometers, particularly the Orbitrap and ion traps.
Key findings:
(1) Semi-tryptic searches are better (2) Use monoisotopic masses for fragment ions [Use monoisotopic masses for precursor ions if data from a high-res instrument] (3) Narrow mass tolerance searches better if search considers precursor mass isotope assignment error
Description:
Advanced tips on using SEQUEST and TPP by Jimmy Eng of U. of Washington, the co-inventor of SEQUEST