"Translational Proteomics 2.0" hosted by Sage-N Research, Inc.
Advanced topics and tips for using SEQUEST and Trans-Proteomic Pipeline tools for proteomics mass spectrometry data analysis.
Proteomics researchers will benefit by increasing their sensitivity and decreasing their false discovery rates when identifying proteins and post-translational modifications using proteomics mass spectrometers, particularly the Orbitrap and ion traps.
(1) Semi-tryptic searches are better
(2) Use monoisotopic masses for fragment ions
[Use monoisotopic masses for precursor ions if data from a high-res instrument]
(3) Narrow mass tolerance searches better if search considers precursor mass isotope assignment error
Advanced tips on using SEQUEST and TPP by Jimmy Eng of U. of Washington, the co-inventor of SEQUEST