Distinct stages of Dictyostelium discoideum life cycle can be seen. As the video starts there is a wave of bacterial growth across the agar....
Distinct stages of Dictyostelium discoideum life cycle can be seen. As the video starts there is a wave of bacterial growth across the agar. From the implantation site of the spores, you can see the myxamoeba migrating out consuming the E.coli. As the E.coli is consumed you can see a clearing in the agar after which the myxamoeba using chemical signaling form multicellular slugs. The slugs can be seen migrating across the field of view, with the formation of a sorocarp in the upper right hand conner of the screen, indicating the end of the life cycle.
Dictyostelium agar was liquified and allowed to solidify inside a Sykes-Moore chamber. E.coli was innoculated onto the agar followed by the implantation of Dictyostelium spores. The Sykes-Moore chamber was assembled and placed under time-lapse videomicrography for three days.