A method for reduction of anesthetic waste gases produced by rodent induction chambers

by: jhlvt
April 21, 2009
DOI: 10.4016/10935.01
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Conference:
American Association for Laboratory Animal Science, Nov. 2008
Abstract:
Isoflurane precision vaporizing systems are generally easy to use and safe for anesthesia of rodents. Our group routinely anesthetized mice and rats for restraint in order to collect blood pressure measurements from the tail. Since mice and rats are difficult to restrain, our laboratory routinely anesthetizes them in an induction chamber then places their nose in a cone for maintenance during a procedure. Ideally, rodents are induced in a chamber and maintained in an area where any waste anesthetic gas (WAG) generated is vented away from the operator by either a ventilated hood or snorkel. Unfortunately, the ideal environment for anesthetizing rodents is not always readily available. Our lab performs experimental procedures that rely on technical equipment; access to in house ventilation was not available. To create a safer environment, we designed a device that reduces WAG and fits in our physical location. Our induction chamber evacuator was designed to blow the anesthetic gas from the chamber before opening, using room air generated via the blower of a small vacuum cleaner. We tested the system using halogenated gas monitoring badges; placing a badge near the opening of the induction chamber and collecting samples from 2.25 to 2.5 hours at a time. Samples were collected with and without the benefit of the added blower. Our research shows that by using the induction chamber evacuator, WAG is reduced by 77.5%. This proved to be an inexpensive and portable devise that can be installed on a variety of different anesthetic systems.
Key findings:
To reduce exposure to anesthetic waste gas coming from induction chambers for laboratory animal handlers anesthetizing rodents.
Description:
Reducing waste gas exposure produced by induction chambers