Chromophore Formation in the Green Fluorescent Protein

submitted by: dgurnon

This animation depicts the reaction of a 3-amino acid sequence within GFP to produce a cyclic chromophore, the source of the protein's fluorescence. The starting conformation and finished cyclic chromophore are representations of actual crystal structures of GFP. Created using UCSF Chimera, Autodesk Maya and Adobe After Effects software.

Aggregation of mouse fetal cells on a SAN nanofiber insert.

submitted by: Arjumond
Visualization of the formation of one large amorphous cell aggregate migrating across the surface of a SAN nanofiber insert. Early passage mouse fetal cells in suspension were injected into a Sykes-Moore chamber containing a SAN nanofiber insert. The cells attached to the nanofiber insert and were observed for seven days using time-lapse videomicrography. Aggregation of a cell monoloayer into one large globular cell aggregate which migrates across the field of view, as observed with 10X...

Fixing Proteomics

submitted by: paddyl
Watch this short 3-minute interview with one of the Fixing Proteomics founders explaining what Fixing Proteomics is trying to do and how you can help. Fixing Proteomics is a non-commercial, technique-independent campaign dedicated to solving the experimental challenges that stop proteomics delivering on its potential. The Fixing Proteomics Campaign is bringing together the people in proteomics who want to tackle the growing frustration and unfair perception that proteomics hasn't...

Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis

submitted by: JCB
The establishment of apical–basal polarity within a single cell and throughout a growing tissue is a key feature of epithelial morphogenesis. To examine the underlying mechanisms, the human intestinal epithelial cell line Caco-2 was grown in a three-dimensional matrix to generate a cystlike structure, where the apical surface of each epithelial cell faces a fluid-filled central lumen. A discrete apical domain is established as early as the first cell division and between the two daughter...
Authors: Aron Jaffe, Noriko Kaji, Joanne Durgan, Alan Hall

One-Dimensional Topography Underlies Three-Dimensional Fibrillar Cell Migration

submitted by: JCB
Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (µPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and...
Authors: Andrew Doyle, Francis Wang, Kazue Matsumoto

Collective cell migration requires vesicular trafficking for chemoattractant delivery at the trailing edge

submitted by: JCB
Chemoattractant signaling induces the polarization and directed movement of cells secondary to the activation of multiple effector pathways. In addition, chemotactic signals can be amplified and relayed to proximal cells via the synthesis and secretion of additional chemoattractant. The mechanisms underlying such remarkable features remain ill defined. We show that the asymmetrical distribution of adenylyl cyclase (ACA) at the back of Dictyostelium discoideum cells, an essential determinant...
Authors: Paul Kriebel, Valarie Barr, Erin Rericha, Guofeng Zhang, Carole Parent