T cell signaling goes with the flow

submitted by: JCB
Activated T cell receptors stimulate actin polymerization at the periphery of the immunological synapses that form between lymphocytes and antigen-presenting cells. Babich et al. reveal that the retrograde flow of actin, largely driven by continuous polymerization, is required to sustain downstream signaling events and T cell activation. This biosights episode presents the paper by Babich et al. from the June 11, 2012 issue of The Journal of Cell Biology and includes an interview with senior...

Coagulation Pathway

submitted by: kkgan

How to study the coagulation cascade

How to grow Yeast

submitted by: Singer Instruments

How to grow / culture yeast on agar or in YPD:

We show you how to prepare fresh cultures of yeast from previously grown agar plates.

Spruce up your old yeast cultures by replating onto fresh media or liquid YPD, or select colonies of interest to be expanded.

Full Yeast Protocol

A new way forward for fibroblasts

submitted by: JCB
Fibroblasts moving across two-dimensional surfaces form lamellipodial protrusions at their leading edge, but how cells move through three-dimensional environments is less well understood. Petrie et al. reveal that, depending on the strength of intracellular RhoA signaling and on the elastic properties of the extracellular matrix, fibroblasts can migrate through 3D environments using either lamellipodia or blunt, cylindrical protrusions called lobopodia. This biosights episode presents the...

How to make Agar

submitted by: Singer Instruments
How to pour YPD Agar plates: This tutorial shows you how we make and pour 400ml of YPD Agar. http://www.singerinstruments.com/index.php?option=com_content&task=view&... Ingredients: 4g Yeast Extract 8g Bacterial Peptone 8g Glucose 6.4g Agar 400ml Water Antibiotic (varies depending on use)

BindingDB: Find my compound's targets

submitted by: gnicpro

This tutorial describes how to discover potentially new targets that may bind your compounds of interest in BindingDB. This unique capability is based on the principle that similar compounds tend to bind the same proteins.