molecular

Jean-Claude Bradley presents on "Peer Review and Science2.0: blogs, wikis and social networking sites" as a guest lecturer for the “Peer Review Culture in Scholarly Publication and Grantmaking” course at Drexel University. The main thrust of the presentation is that peer review alone is not capable of coping with the increasing flood of scientific information being generated and shared. Arguments are made to show that providing sufficient proof for scientific findings does scale and...

Intestinal roundworms infect >2 billion of the poorest peoples and have received little attention. Very little money is spent on much needed drug development for human parasites, making them one of the great neglected tropical diseases. Recently, a new and powerful class of anti-roundworm drugs (anthelmintic) has emerged- crystal proteins (e.g., Cry5B) made by Bacillus thuringiensis (Bt). Here we show that combining Cry5B with nicotinic acetylcholine receptor agonists like tribendimidine and...

Simon Prochnik spoke about the Naegleria gruberi genome project at the DOE JGI on February 3, 2010.

by Abnova - Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles.

by Abnova - Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein.

by Abnova - Transfected lysate is useful as a positive control in Western Blot. The full-length protein construct is expressed in HEK293T cells and prepared as ready-to-use, denatured lysate in 1x Sample Buffer.