education
SDSU Geological Sciences Webinar - Stephen Self
submitted by: tcarrasc
Super-eruptions: Volcanic Activity with a Global Impact; Stephen Self, Volcano Dynamics Group, Department of Earth Science, The Open University, Senior Volcanologist, US NRC - Every now and again, Earth suffers from tremendous explosive volcanic eruptions, much bigger than those witnessed in modern times. Although the return period for such events is long, perhaps every 10-100,000 years depending on the size, it is statistically more likely that Earth will next experience a large...
Do objects have weight in space? - Take your classroom into space
submitted by: cristina
What's the difference between mass and weight? Do objects have weight in space? Students using ESAs 'Take Your Classroom into Space' kit and this video recorded in the unique environment of the ISS can now find out. The experiment was proposed by Greek, Belgian and Spanish teachers who participated to the ESA competition 'Take Your Classroom into Space'. http://www.esa.int/esaHS/education.html
Exploring capillarity - Take your classroom into space
submitted by: cristina
Gravity has something to do with capillary action, but what? Students using ESAs 'Take Your Classroom into Space' kit and this video recorded in the weightlessness envirnoment of the ISS can now find out. The experiment was proposed by a Belgian and Italian teachers who participated to the ESA competition 'Take Your Classroom into Space'.
http://www.esa.int/esaHS/education.html
Save our Center: Inventory of Metrics for the California Center for the Arts, Escondido
submitted by: datrujil
GST Enzymatic Digestion
submitted by: abnova
By Abnova - GST fusion proteins allow convenient affinity purification of many proteins of interest but the GST-tag may become inconvenient in various downstream applications. We use PreScission protease to digest the GST fusion proteins and the protein without GST-tag is obtained.
Protein Quantification (Bradford Assay)
submitted by: abnova
By Abnova - Bradford assay is a rapid and accurate method to determine the concentration of protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mcg/ml), the more protein present, the more Coomassie binds.