Dennis Hedgecock at 2010 DOE JGI User Meeting

submitted by: JGI

Dennis Hedgecock from the University of Southern California answers the question, "Genomics on the Half Shell: So, What Do Oysters Have to Do with Energy?" on March 24, 2010 at the 5th Annual DOE JGI User Meeting.

Evan DeLucia at 2010 DOE JGI User Meeting

submitted by: JGI

Evan DeLucia of the University of Illinois at Urbana-Champaign and the Energy Biosciences Institute talks about "The Promise and Challenge of Producing Biofuel Feedstocks: An Ecological Perspective" on March 25, 2010 at the 5th Annual DOE JGI User Meeting.

mRNA Extraction

submitted by: abnova
Abnova - Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA. For more videos, visit Abnova !

Real-time PCR

submitted by: abnova

Abnova - Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. For more videos, visit Abnova!

Reverse Transcription PCR

submitted by: abnova

Abnova - Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR. For more videos, visit Abnova!

RNA Extraction from Cell Culture

submitted by: abnova

Abnova - The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology. This video shows how to extract total RNA from cell culture. For more videos, visit Abnova!