Antibody Purification (Affinity)

submitted by: abnova
Abnova - Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix. For more videos, visit Abnova !

Indirect ELISA

submitted by: abnova
Abnova - The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody (direct detection) and conjugated secondary antibodies (indirect detection). For more videos, visit Abnova !

Joseph Noel at 2010 DOE JGI User Meeting

submitted by: JGI

Joseph Noel from the Salk Institute on "Metabolic Noise, Vestigial Metabolites or the Raw Material of Ecological Adaptation? Enzymes, Catalytic Promiscuity and the Evolution of Chemodiversity in Nature" on March 26, 2010 at the 5th Annual DOE JGI User Meeting.

Adrian Tsang at the 2010 DOE JGI User Meeting

submitted by: JGI

Adrian Tsang from Concordia University on "Fungal Decomposition of Lignocellulosic Substrates" on March 26, 2010 at the 5th Annual DOE JGI User Meeting.

Tanja Woyke at the 2010 DOE JGI User Meeting

submitted by: JGI

Tanja Woyke from the DOE JGI on the "Genomic Sequencing of Single Bacterial Cells" on March 26, 2010 at the 5th Annual DOE JGI User Meeting.

Steve Savage at the 2010 DOE JGI User Meeting

submitted by: JGI

Steve Savage from Cirrus Partners on "Three Non-Technical Challenges in the Development of Biomass-based Energy" on March 25, 2010 at the 5th Annual DOE JGI User Meeting.