Uffe Hellsten on the first frog genome, X. tropicalis

submitted by: JGI

DOE JGI's Uffe Hellsten, first author of the April 30, 2010 Science publication about the first amphibian genome to be sequenced, talks about the importance of the African clawed frog.

Antibody Conjugation-Biotinylation

submitted by: abnova

Abnova - Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. A biotin/streptavidin (avidin) system offers low background, enhanced sensitivity and the ability to detect minute amounts of antigens. For more videos, visit Abnova!

Antibody Conjugation-FITC

submitted by: abnova

Abnova - The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability. For more videos, visit Abnova!

Antibody Purification (Affinity)

submitted by: abnova
Abnova - Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix. For more videos, visit Abnova !

Indirect ELISA

submitted by: abnova
Abnova - The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody (direct detection) and conjugated secondary antibodies (indirect detection). For more videos, visit Abnova !

Joseph Noel at 2010 DOE JGI User Meeting

submitted by: JGI

Joseph Noel from the Salk Institute on "Metabolic Noise, Vestigial Metabolites or the Raw Material of Ecological Adaptation? Enzymes, Catalytic Promiscuity and the Evolution of Chemodiversity in Nature" on March 26, 2010 at the 5th Annual DOE JGI User Meeting.