Cell Lysate Preparation (Native)

submitted by: abnova

Abnova - This protocol involves three steps to prepare cell lysate. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as read-to-use, native lysate in modified RIPA Buffer. For more videos, visit Abnova!

Cell Lysate Preparation (Denatured)

submitted by: abnova

Abnova - This protocol involves three steps to prepare cell lysate. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as read-to-use, denatured lysate in 1x Sample Buffer. For more videos, visit Abnova!

Why Science Is Cool_MacCartney

submitted by: amandamac

For the Kavli Science Video Contest with numerous fields of science explored that exist in our lives everyday without our acknowledgement.

AsiGrun - Green Paper Reciepts

submitted by: PuzzekA

Look at the waste of paper in our everyday life...
Every purchase we make we get a useless paper receipt.
we suggest a green solution instead the traditional use of paper receipts!

Mononuclear Cell Isolation from Whole Blood

submitted by: abnova
Abnova - This procedure to isolate mononuclear cells from whole blood is based on density differences between mononuclear cells and other elements found in the blood sample. Differential migration during centrifugation results in the formation of layers containing different cell types. Mononuclear cells are at the interface between the plasma and the ficoll layer and are recovered by washing steps. For more videos, visit Abnova !

Cell Nuclei Protein Extraction

submitted by: abnova

Abnova - This protocol involves three steps to prepare nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer. For more videos, visit Abnova!