His-tagged Protein Purification

submitted by: abnova
( http://www.abnova.com ) - Proteins with histidine tag can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This video provides generic protocol to purify 6xHis-tagged protein by Nickel nitrilotriacetic (Ni-NTA) sepharose. More videos at Abnova !

Gene ORF Finder

submitted by: abnova

( http://www.abnova.com ) - ORF Finder identifies all possible ORFs in a DNA sequence by locating the standard and alternative stop and start codons. The deduced amino acid sequences can then be used to BLAST against GenBank. More videos at Abnova!

Cell Counting - Cellometer®

submitted by: abnova

( http://www.abnova.com ) - The Nexcelom Bioscience Cellometer® is a simple to use, automated cell counter designed to improve accuracy and repeatability. You just pipette 20µL of sample, insert the slide and click count. Cell Concentration, cell size and viability are displayed in less than 30 seconds. More videos at Abnova.

UV-Vis Spectrophotometer - Nanodrop®

submitted by: abnova

( http://www.abnova.com ) - The Thermo Scientific NanoDrop® is a full-spectrum spectrophotometer that measures 1 ul samples with high accuracy and reproducibility. It utilizes a sample retention technology that eliminates the need for cumbersome cuvettes and allows for clean up in seconds. More videos at Abnova.

DNA Gel Preparation

submitted by: abnova

Abnova - Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the agarose gel. More videos at Abnova.

Protein Structure

submitted by: abnova

Abnova - "Entrez Structure", also known as Molecular Modeling DataBase (MMDB), is a database of experimentally determined structures obtained from the RCSB Protein Data Bank (PDB). It provides a wealth of information on the biological function, on mechanisms linked to the function, and on the evolutionary history of relationships between macromolecules. More videos at Abnova!