Protemist® XE

submitted by: abnova

Abnova - Protemist® XE is a fully-automated desktop protein synthesizer for large scale protein production using the wheat germ cell-free expression system. It is ideally suited for producing 10 mg to over 500 mg of protein for structural analysis, functional analysis, and assay development. More videos at Abnova!

SDS-PAGE Preparation

submitted by: abnova

Abnova - SDS-PAGE is a method used to separate proteins according to their size. This video shows you how to prepare SDS-PAGE with two layers of gel, separating gel and stacking gel. More videos at Abnova Abnova!

Gene Conserved Domains

submitted by: abnova
Abnova - Conserved domains database (CDD) is a protein annotation resource that consists of a collection of well-annotated multiple sequence alignment models for ancient domains and full-length proteins. These are available as position-specific score matrices (PSSMs) for fast identification of conserved domains in protein sequences via RPS-BLAST (Reverse Position-Specific). More videos at Abnova !

BLAST - Protein

submitted by: abnova
Abnova - BLAST-Protein (blastp) is used for both identifying a query amino acid sequence and for finding similar sequences in protein databases. Like other BLAST programs, blastp is designed to find local regions of similarity. When sequence similarity spans the whole sequence, blastp will also report a global alignment, which is the preferred result for protein identification purposes. More videos at Abnova !

BLAST - PCR Primers Design

submitted by: abnova

Abnova - Primer-BLAST was developed to help users make primers that are specific to the input PCR template. It uses Primer3 to design PCR primers and then submits them to BLAST search against user-selected database. The blast results are then automatically analyzed to avoid primer pairs that can cause amplification of targets other than the input template. More videos at Abnova !

BLAST - Multiple Alignment

submitted by: abnova

Abnova - Multiple sequence alignment is an extension of pairwise alignment to incorporate more than two sequences at a time. It is often used to identify conserved sequence regions which are assumed to be evolutionarily related. By construct phylogenetic trees, it aids in establishing evolutionary relationships. More videos at Abnova !