Dr Kerstin Strupat presents her scientific poster about singly charged AP MALDI-produced ions at high mass range using the Thermo Scientific Exactive Plus mass spectrometer. For the first time, intact bovine insulin at m/z 5734 is detected on an Exactive series mass spectrometer.

Jonathan Beck discusses online sample pre-concentration and extraction of water samples followed by analysis with high-resolution, accurate mass (HR/AM) detection, quantitation and confirmation. Screening, confirmation and quantitation of antibiotics in drinking water was done in the same analytical run with the Thermo Scientific Orbitrap platform.

John Rogers presents his scientific poster about human cell-free expression system for the production of stable-isotope-labeled (heavy) proteins using HeLa cell lysates. Six different stable-isotope-labeled proteins were produced. Isotope incorporation efficiency of greater than 95% was observed for IVT reactions.

Iman Mohtashemi presents his scientific poster about node-based framework for proteomics data processing using Thermo Scientific Proteome Discoverer software and a hybrid ion trap-OribtrapTM mass spectrometer. This allows rapid deployment of custom algorithms without the need to develop graphical user interfaces and other input/output infrastructure saving you time.

David Horn presents his scientific poster on the necessity for high mass resolution (>200,000) for confidence in identification of proteins above 40kDa using yeast enolase. The comparison between various resolution settings for both direct infusion and LC-MS/MS data are shown, using the Thermo Scientific Orbitrap mass spectrometer to improve confidence and sequence coverage for identification of larger proteins.

Documentation of the seminar on by Dr. Carlito R. Barril, Retired Professor of Chemistry of the University of the Philippines Los Baños during the Special Seminar on Fishkill last August 24, 2012 at the UPLB Operations Room, AG Samonte Hall, UPLB.