Silencing the Menkes Copper-Transporting ATPase (Atp7a) Gene in Rat Intestinal Epithelial (IEC-6) Cells Increases Iron Flux via Transcriptional Induction of Ferroportin 1 (Fpn1)

submitted by: jfcollins
The Menkes copper-transporting ATPase (Atp7a) gene is induced in rat duodenum during iron deficiency, consistent with copper accumulation in the intestinal mucosa and liver. To test the hypothesis that ATP7A influences intestinal iron metabolism, the Atp7a gene was silenced in rat intestinal epithelial (IEC-6) cells using short hairpin RNA (shRNA) technology. Perturbations in intracellular copper homeostasis were noted in knockdown cells, consistent with the dual roles of ATP7A in pumping...
Authors: Sukru Gulec, James F. Collins

biosights: Decemeber 23, 2013 - Tumor cells WASH away the extracellular matrix

submitted by: JCB
Tumor cells invade through extracellular matrices by forming actin-rich structures called invadopodia, which contain the transmembrane matrix metalloproteinase MT1-MMP. Monteiro et al. reveal that the Arp2/3 activating protein WASH works with the exocyst complex to deliver MT1-MMP from late endosomes to the invadopodial plasma membrane. This biosights episode presents the paper by Monteiro et al. from the December 23, 2013, issue of The Journal of Cell Biology and includes an interview with...

The addition of soft tissue replacement grafts in plastic periodontal and implant surgery: critical elements in design and execution

submitted by: Otto Zuhr
Soft tissue replacement grafts have become a substantial element to increase tissue volume in plastic periodontal and implant surgery. Autogenous subepithelial connective tissue grafts are increasingly applied in aesthetic indications like soft tissue thickening, recession treatment, ridge preservation, soft tissue ridge augmentation and papilla reconstruction. For the clinical performance of connective tissue graft harvesting and transplantation, a fundamental understanding of the anatomy...
Authors: Daniel Bäumer, Markus Hürzeler, Otto Zuhr

biosights: November 25, 2013 - Caspases work as branch managers

submitted by: JCB
When a growing axon nears its target, it arborizes, or branches, to form numerous synaptic connections. Campbell and Okamoto reveal that localized caspase activation downstream of Slit-Robo signaling promotes arbor dynamics and restricts arbor growth. This biosights episode presents the paper by Campbell and Okamoto from the November 25, 2013, issue of The Journal of Cell Biology and includes an interview with lead author Douglas Campbell (formerly at the RIKEN Brain Science Institute,...

MWV #81 - Sheldon Campbell - The Singing Microbiologist

submitted by: MicrobeWorld
Sheldon Campbell sings about microbiology. Dr. Campbell teaches microbiology at Yale School of Medicine and he uses music to enhance his lectures. He has one song for every block of lectures he gives on a major topic. Songs he's written include a song about fungi, tick borne disease, tuberculosis and one that reviews all of microbiology in eight minutes. Dr. Campbell hasn't done any testing to see his songs are more effective at getting his message across but he does get the occasional...

Extraction sockets: erratic healing impeding factors

submitted by: freetist
Aim: The aim of this exploratory study was to analyse prevalence of extraction sockets showing erratic healing and evaluate factors potentially impeding healing. Methods: Erratic healing was defined as extraction sites showing clinical observations of fibrous scar tissue occupying the extraction site rather than bone following 12 or more weeks of healing. Computerized tomography was used to evaluate characteristics and calculate Hounsfield unit scores for sites showing erratic healing....
Authors: Jung-Hoon Kim, Cristiano Susin, Jeong-Hyun Min, Hee-Yeon Suh, Eun-Jung Sang, Young Ku, Ulf M. E. Wikesjo, Ki-Tae Koo