biochemistry

Theory and endeavors in the physical, biological and anthropological sciences are contrasted in order to discuss validity in the representation of nature. A physical model for the evolution of knowledge is presented that equates the universe with the entity path; the conceptual and physical, memory and path, the immaterial and the material, respectively as the effect of path. The universe, infinite in inexact description only, is structurally reframed employing the orientation of the...

By Abnova - GST fusion proteins allow convenient affinity purification of many proteins of interest but the GST-tag may become inconvenient in various downstream applications. We use PreScission protease to digest the GST fusion proteins and the protein without GST-tag is obtained.

By Abnova - Bradford assay is a rapid and accurate method to determine the concentration of protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mcg/ml), the more protein present, the more Coomassie binds.

By Abnova - Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity while using very simple and cheap equipment and chemicals. The procedures of silver staining are protein fixation, silver impregnation and image development.

by Abnova - A powerful platform for protein-protein interaction and protein phosphorylation studies in situ is created by combining Abnovas dual recognition, primary antibody pair with proximity ligation assay from Olink Bioscience. This revolutionary combination enables unprecedented specificity and sensitivity of protein detection and quantification for high performance immunofluorescence and immunohistochemistry applications.

by Abnova - Cell culture is the process by which cells are grown under controlled conditions. This video show you how to culture suspension cells from cell thrawing, maintain, to cell freezing.